Analysis of Cys aminoacyl synthetase.

Analysis performed on 2024-07-10 11:38:18

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 46644 573 46071 26574
Archaea 3232 83 3149 1281
Vertebrata 1844 685 1159 434
Mammals 862 201 661 128

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
748 11 623

Domains

Domains
Current Study 1, 20 | 644, 749
Guo et al (2010) 1, 82 | 201, 297 | 664, 748

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 623 125 748 4.984000
Number of Variants 522 112 634 4.660714
Number of Pathogenic Variants 9 2 11 4.500000
Number of Benign/Unknown Variants 513 110 623 4.663636
Fisher exact test 1.000000

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 484 264 748 1.833333
Number of Variants 415 219 634 1.894977
Number of Pathogenic Variants 10 1 11 10.000000
Number of Benign/Unknown Variants 405 218 623 1.857798
Fisher exact test 0.108010

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 623 125 0.81 (0.01) 0.81 (0.01) 8.051e-01 8.764e-01 40745.00 2.06e-01
Guo et al (2010) 484 264 0.80 (0.01) 0.81 (0.01) 8.031e-01 8.274e-01 62504.50 6.88e-01

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains